Abstract

This study was conducted to test the feasibility of the growth of the plant in terms terms of tissue culture, callus formation specifically. From the original Murashige and Skoog Basal Medium, it was modified and three other treatments were made labelled as T1, T2, T3 and T4 for the controlled set-up. Treatments varied in the concentraation of cytokinins 6-benzyladinine(BA), and auxins 1-Napthaleneacetic acid (NAA) and 1H-indole-3-butyric acid (IBA).

Half of the treatments were placed inside the dark room and the other half exposed to sunlight during the day and artificial light at night. The set-ups were set under observation for a total of 56 days and observations were made every after 2 weeks. Results showed that among all the four treatments observed, T2 had the best performance in terms of callus initiation. It produced the biggest callus among all in both the light and dark conditions followed by T1, T4 and T3, respectively. Based on the findings, T2 is the best among all the treatments in growing of explants.